Objectives


Despite increasing bodies of proofs have been gained on graphene intriguing physical, chemical and mechanical properties, safety considerations such as the assessment of the immunological impact of graphene on human and environmental organisms are still under-investigated.

Similarly, many investigations have paid attentions to GFM biocompatibility, but the potential genotoxic impact has been explored to a less extent. Indeed, due to its unique layered nanostructure, graphene can interact with DNA through DNA-intercalation and cleavage, displaying mutagenic effect both in vitro and in vivo, as well as flake size-dependent genotoxicity.

The specific scientific and technological objectives of G-IMMUNOMICS are to:

  1. Produce highly stable and dispersible pristine and functionalized GFMs such as Exfoliated Graphene (G) and Graphene Oxide (GO) with different lateral size and appropriate functionalizations;
  2. Characterize through high throughput functional immunogenomics and proteomics approaches the immune cell response induced by functionalized graphene on different cell lines (from human, mouse and swine) and primary cells (human, swine, mouse and nematodes);
  3. Evaluate the genotoxic effects of the selected materials onĀ  different species: nematodes (C. elegans) and mammalians (M. musculus, S. scrofa and human).

Objectives